Percp type component
The peridinin-chlorophyll-protein complex (PCP or PerCP) is a soluble molecular complex consisting of the peridinin-chlorophyll a-protein bound to peridinin, chlorophyll, and lipids. The peridinin molecules absorb light in the blue-green wavelengths (470 to 550 nm) and transfer energy to the chlorophyll molecules with extremely high efficiency. PCP complexes are found in many p. StructureThe PCP protein has been identified in dinoflagellate in at least two forms, a homodimeric form composed of two 15- monomers, and a monomeric form of around 32kD believed to have evolved from the homodi. . contain -bound similar to those found in . They additionally contain water-soluble protein-pigment complexes that exploit. [PDF Version]FAQS about Percp type component
How many peridinin & chlorophyll are in a PerCP complex?
The PerCP complex has a high ratio of peridinin to chlorophyll; most light-harvesting complexes contain more chlorophyll than carotenoids, but the PerCP contains eight peridinin and two chlorophyll molecules arranged to promote peridinin-chlorophyll energy transfer. The PerCP complex is a dimer with a molecular weight of approximately 35kDa.
What is PerCP fluorescent labeling?
As the leading commercial form of PCPs, PerCP fluorescent labeling is widely applied in medical research . The commercial PerCP complex has a molecular weight of 35 kDa, a broad excitation spectrum (maximum adsorption: 472–488 nm), and a maximum emission around 676 nm . The absorption and emission spectrum are shown in Fig. 2 c.
What is PerCP used for in research applications?
PerCP is commonly used in research applications such as fluorescence-activated cell sorting (FACS) and flow cytometry. The fluorophore is covalently linked to proteins or antibodies for use in these techniques. Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
What is a PerCP conjugate in Dinophyceae SP?
Jackson ImmunoResearch offers the form found in Dinophyceae sp. It has a broad spectrum of excitation with the main peak at 482 nm, and a long Stokes shift to an emission peak at 677 nm. PerCP conjugates are large complexes suitable for cell surface labeling techniques such as flow cytometry.